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blyscan dye reagent  (Heidolph Instruments)

 
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    Heidolph Instruments blyscan dye reagent
    Blyscan Dye Reagent, supplied by Heidolph Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blyscan+dye+reagent/blyscan+dye+reagent/bio_rxiv__699496-199-19-29
    Average 90 stars, based on 1 article reviews
    blyscan dye reagent - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Effects of amotosalen treatment on human platelet lysate bioactivity
    Article Snippet: 100 μL of each standard and sample was then added to the new micro tubes and mixed with the Blyscan dye reagent for 30 minutes on a mechanical shaker (Heidolph, Schwabach, Germany).



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    Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
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    Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
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    Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
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    Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
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    Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
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    Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
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    blyscan dye reagent (biocolor, uk)  (Biocolor Ltd)
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    Radiation stimulates vesicular CD44v3 expression. (A, <t>B)</t> <t>tEVs</t> from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via <t>blyscan</t> assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.
    Blyscan Dye Reagent (Biocolor, Uk), supplied by Biocolor Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blyscan+dye+reagent/blyscan+dye+reagent/us09956251-157-0-3
    Average 90 stars, based on 1 article reviews
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    Radiation stimulates vesicular CD44v3 expression. (A, B) tEVs from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via blyscan assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.

    Journal: Frontiers in Oncology

    Article Title: Radiation induces ESCRT pathway dependent CD44v3 + extracellular vesicle production stimulating pro-tumor fibroblast activity in breast cancer

    doi: 10.3389/fonc.2022.913656

    Figure Lengend Snippet: Radiation stimulates vesicular CD44v3 expression. (A, B) tEVs from breast cancer cells were assessed for CD44v3 (A) and HS (B) expression via flow cytometry. Control represents unbound beads incubated with antibody cocktail. (C) tEVs from MDA-MB-231 cells were treated overnight with either heparanase, chondroitinase ABC, or both. Gag levels were assessed via blyscan assay. Data points represent technical replicates. (D) MRC5 were incubated with tEVs collected from WT or CD44 KO MDA-MB-231. IL-6 levels were measured via ELISA. (E) MDA-MB-231 were exposed to fresh media containing FCM described in (D) and radioresistance was assessed via clonogenic assay. X axis indicates which fibroblast CM from (D) was used in the incubation. Statistical significance for (A-E) assessed with ANOVA * = P < 0.05. (D, E) Data points represent biological replicates. ** denotes a P value < 0.005. *** denotes a P value < 0.0005. **** denotes a P value < 0.00005. These values were the results of ANOVA analyses performed on GraphPad. “ns” represents a value for P that is greater than 0.05 and stands for “Not Significant”.

    Article Snippet: tEVs (50 mg) were stained with 1mL blyscan dye reagent for 30 minutes according to the manufacturers protocol (BioVendor R&D, Asheville, NC).

    Techniques: Expressing, Flow Cytometry, Control, Incubation, Enzyme-linked Immunosorbent Assay, Clonogenic Assay